Nucleic Acid Extraction
The basis of HandyLab’s DNA and RNA Nucleic Acid Extraction technology is the proprietary affinity molecules that offer a very high density of positively ionizable charges at a low pH. These enable strong attraction and binding of polynucleotides from a clinical lysate within a few minutes and yield excellent nucleic acid recovery. The technology can be applied to a wide variety of sample matrices, both clinical and non-clinical.
Both the Manual and Automated Extraction procedures use a combination of lytic and extraction reagents to perform cell lysis, DNA or RNA extraction and removal of inhibitors. Following cell lysis, the released nucleic acid is captured by magnetic affinity beads.
The beads, with the bound nucleic acids, are washed and the nucleic acid is eluted
using ~10 µL of release solution and prepared for PCR by adding an equal volume of neutralization reagent. On the Lynx system, additional release and neutralization reagents are added according to the desired final volume. |
Microfluidic Real-Time PCR
HandyLab has created breakthrough technologies for rapid, microfluidic real-time PCR. This technology is incorporated into the Jaguar system. PCR reactions are performed in disposable microfluidic cartridges and thermocycled by microthermal circuits that allow cycling times as low as 20 seconds to be achieved. This enables 45 cycles to be completed in 11-15 minutes.
An ultrasensitive fluorescence-detection system enables real-time PCR detection from the microvolumes (4-5 µl) present in the cartridge reactors. These 12 reactors are individually controlled allowing for maximum flexibility in running different protocols.
The reaction cartridges have microvalves which contain a thermally responsive wax. These microvalves are sealed at the start of the PCR process which minimizes evaporation and the risk of contamination. |
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